Full metadata
Title
Optimization of a Blood-Based Tuberculosis Diagnostic Assay for the Developing World-with a focus on Sub-Saharan Africa
Description
The optimization of a blood-based assay for diagnosing tuberculosis which has been developed and validated in Dr. Hu’s lab, at Arizona State University, is important for ensuring its successful translation to a resource-limited setting of the developing world. Tuberculosis is most prevalent in the developing world with Sub-Saharan Africa having the highest cases of HIV/TB coinfections. The implementation of a blood-based assay for diagnosing Tuberculosis in the sub-Saharan would significantly improve the diagnosis and treatment monitoring of tuberculosis thereby managing or eliminating the pandemic altogether. The World Health Organization has called for robust diagnostic technologies that would resolve the shortfalls of the current technologies which include GeneXpert, X-ray, and smear microscopy. The blood-based diagnostic methodology heavily relies on Mass-spectrometry, a technology which could be entirely novel and expensive to implement in most laboratories in the Sub-Saharan. Despite virtual challenges in implementing the technology, the assay has demonstrated high specificity and sensitivity to HIV/TB coinfected patients and children in comparison to the available TB diagnostic assays. This study endorses the Blood-based Mass Spectrometry assay as one of the promising technologies to effectively improve the diagnosis of TB. The performance of the assay on detecting TB antigens was tested using different methods and materials. In the end, the use of DBS and miniaturized mass spectrometers have been discussed as possible routes for translating the assay to the developing world
Date Created
2019-05
Contributors
- Twaibu, Jaffalie (Author)
- Hu, Tony (Thesis director)
- Shu, Qingbo (Committee member)
- Harrington Bioengineering Program (Contributor)
- Barrett, The Honors College (Contributor)
Topical Subject
Resource Type
Extent
38 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2018-2019
Handle
https://hdl.handle.net/2286/R.I.53045
Level of coding
minimal
Cataloging Standards
System Created
- 2019-04-24 12:00:07
System Modified
- 2021-08-11 04:09:57
- 3 years 3 months ago
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