Full metadata
Title
Construction of Recombinant Plasmids of Corynebacterium glutamicum for Flavonoid Production
Description
Due to the wide range of health properties flavonoids possess, flavonoids are sold in health supplements to the general public. Flavonoids are also utilized in research but have a high cost due to current production techniques. This project focuses on engineering two DNA recombinants to develop new strains of Corynebacterium glutamicum that can produce flavonoids pinocembrin and naringenin. After culturing Escherichia coli colonies containing genes of interest, the genes were collected and purified by PCR reactions. The recombinant plasmid was assembled using CPEC and successfully transformed into Escherichia coli, with plans to transform Corynebacterium glutamicum to experiment and determine which recombinant can produce more pinocembrin and naringenin. Design work for other DNA recombinants, which were not the focus of this project, was also completed.
Date Created
2019-05
Contributors
- Wong, Adam (Co-author, Co-author)
- Varman, Arul Mozhy (Thesis director)
- Nielsen, David (Committee member)
- Chemical Engineering Program (Contributor)
- Barrett, The Honors College (Contributor)
Topical Subject
Resource Type
Extent
22 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2018-2019
Handle
https://hdl.handle.net/2286/R.I.53357
Level of coding
minimal
Cataloging Standards
System Created
- 2019-05-04 12:40:15
System Modified
- 2021-08-11 04:09:57
- 3 years 2 months ago
Additional Formats