Description
Using a CH[subscript 4]-based membrane biofilm reactor (MBfR), we studied perchlorate (ClO[subscript 4]–) reduction by a biofilm performing anaerobic methane oxidation coupled to denitrification (ANMO-D). We focused on the effects of nitrate (NO[subscript 3]–) and nitrite (NO[subscript 2]–) surface loadings on ClO[subscript 4]– reduction and on the biofilm community’s mechanism for ClO[subscript 4]– reduction. The ANMO-D biofilm reduced up to 5 mg/L of ClO[subscript 4]– to a nondetectable level using CH[subscript 4] as the only electron donor and carbon source when CH[subscript 4] delivery was not limiting; NO[subscript 3]– was completely reduced as well when its surface loading was ≤0.32 g N/m[superscript 2]-d. When CH[subscript 4] delivery was limiting, NO[subscript 3]– inhibited ClO[subscript 4]– reduction by competing for the scarce electron donor. NO[subscript 2]– inhibited ClO[subscript 4]– reduction when its surface loading was ≥0.10 g N/m[superscript 2]-d, probably because of cellular toxicity. Although Archaea were present through all stages, Bacteria dominated the ClO[subscript 4]–-reducing ANMO-D biofilm, and gene copies of the particulate methane mono-oxygenase (pMMO) correlated to the increase of respiratory gene copies. These pieces of evidence support that ClO[subscript 4]– reduction by the MBfR biofilm involved chlorite (ClO[subscript 2]–) dismutation to generate the O[subscript 2] needed as a cosubstrate for the mono-oxygenation of CH[subscript 4].
Included in this item (3)
Permanent Link
Permanent Link
Details
Title
- Complete Perchlorate Reduction Using Methane as the Sole Electron Donor and Carbon Source
Contributors
Agent
- Luo, Yi-Hao (Author)
- Chen, Ran (Author)
- Wen, Li-Lian (Author)
- Meng, Fan (Author)
- Zhang, Yin (Author)
- Lai, Chun-Yu (Author)
- Rittmann, Bruce (Author)
- Zhao, He-Ping (Author)
- Zheng, Ping (Author)
- Biodesign Institute (Contributor)
- Swette Center for Environmental Biotechnology (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-02-17
Collections this item is in
Identifier
-
Digital object identifier: 10.1021/es504990m
-
Identifier TypeInternational standard serial numberIdentifier Value0013-936X
-
Identifier TypeInternational standard serial numberIdentifier Value1520-5851
Note
-
This document is the Accepted Manuscript version of a Published Work that appeared in final form in
ENVIRONMENTAL SCIENCE & TECHNOLOGY, copyright American Chemical Society, after peer review and technical editing by the publisher.
To access the final published work, see http://dx.doi.org/10.1021/es504990m
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Luo, Yi-Hao, Chen, Ran, Wen, Li-Lian, Meng, Fan, Zhang, Yin, Lai, Chun-Yu, Rittmann, Bruce E., Zhao, He-Ping, & Zheng, Ping (2015). Complete Perchlorate Reduction Using Methane as the Sole Electron Donor and Carbon Source. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 49(4), 2341-2349. http://dx.doi.org/10.1021/es504990m