Description
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Details
Title
- The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
Contributors
- Edlund, Petra (Author)
- Takala, Heikki (Author)
- Claesson, Elin (Author)
- Henry, Leocadie (Author)
- Dods, Robert (Author)
- Lehtivuori, Heli (Author)
- Panman, Matthijs (Author)
- Pande, Kanupriya (Author)
- White, Thomas (Author)
- Nakane, Takanori (Author)
- Berntsson, Oskar (Author)
- Gustavsson, Emil (Author)
- Bath, Petra (Author)
- Modi, Vaibhav (Author)
- Roy Chowdhury, Shatabdi (Author)
- Zook, James (Author)
- Berntsen, Peter (Author)
- Pandey, Suraj (Author)
- Poudyal, Ishwor (Author)
- Tenboer, Jason (Author)
- Kupitz, Christopher (Author)
- Barty, Anton (Author)
- Fromme, Petra (Author)
- Koralek, Jake D. (Author)
- Tanaka, Tomoyuki (Author)
- Spence, John (Author)
- Liang, Mengning (Author)
- Hunter, Mark S. (Author)
- Boutet, Sebastien (Author)
- Nango, Eriko (Author)
- Moffat, Keith (Author)
- Groenhof, Gerrit (Author)
- Ihalainen, Janne (Author)
- Stojkovic, Emina A. (Author)
- Schmidt, Marius (Author)
- Westenhoff, Sebastian (Author)
- College of Liberal Arts and Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2016-10-19
Resource Type
Collections this item is in
Identifier
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Digital object identifier: 10.1038/srep35279
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Identifier TypeInternational standard serial numberIdentifier Value2045-2322
Note
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The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep35279
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Edlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., . . . Westenhoff, S. (2016). The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. Scientific Reports, 6(1). doi:10.1038/srep35279