Description
Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.
Details
Title
- X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex
Contributors
- Zhou, X. Edward (Author)
- Gao, Xiang (Author)
- Barty, Anton (Author)
- Kang, Yanyong (Author)
- He, Yuanzheng (Author)
- Liu, Wei (Author)
- Ishchenko, Andrii (Author)
- White, Thomas A. (Author)
- Yefanov, Oleksandr (Author)
- Han, Gye Won (Author)
- Xu, Qingping (Author)
- de Waal, Parker W. (Author)
- Suino-Powell, Kelly M. (Author)
- Boutet, Sebastien (Author)
- Williams, Garth J. (Author)
- Wang, Meitian (Author)
- Li, Dianfan (Author)
- Caffrey, Martin (Author)
- Chapman, Henry N. (Author)
- Spence, John (Author)
- Fromme, Petra (Author)
- Weierstall, Uwe (Author)
- Stevens, Raymond C. (Author)
- Cherezov, Vadim (Author)
- Melcher, Karsten (Author)
- Xu, H. Eric (Author)
- College of Liberal Arts and Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2016-04-12
Resource Type
Collections this item is in
Identifier
- Digital object identifier: 10.1038/sdata.2016.21
- Identifier TypeInternational standard serial numberIdentifier Value2052-4463
Note
- The final version of this article, as published in Scientific Data, can be viewed online at: https://www.nature.com/articles/sdata201621
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Zhou, X. E., Gao, X., Barty, A., Kang, Y., He, Y., Liu, W., . . . Xu, H. E. (2016). X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. Scientific Data, 3, 160021. doi:10.1038/sdata.2016.21