Description
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of

Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
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    Title
    • Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
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    Date Created
    2015-01-27
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    Identifier
    • Digital object identifier: 10.1107/S2052252514026487
    • Identifier Type
      International standard serial number
      Identifier Value
      2052-2525
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    • View the article as published at http://journals.iucr.org/m/issues/2015/02/00/jt5008/index.html

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    Nogly, P., James, D., Wang, D., White, T. A., Zatsepin, N., Shilova, A., . . . Weierstall, U. (2015). Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. IUCrJ, 2(2), 168-176. doi:10.1107/s2052252514026487

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