Description
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 Å. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
Details
Title
- Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
Contributors
- Nogly, Przemyslaw (Author)
- James, Daniel (Author)
- Wang, Dingjie (Author)
- White, Thomas A. (Author)
- Zatsepin, Nadia (Author)
- Shilova, Anastasya (Author)
- Nelson, Garrett (Author)
- Liu, Haiguang (Author)
- Johansson, Linda (Author)
- Heymann, Michael (Author)
- Jaeger, Kathrin (Author)
- Metz, Markus (Author)
- Wickstrand, Cecilia (Author)
- Wu, Wenting (Author)
- Bath, Petra (Author)
- Berntsen, Peter (Author)
- Oberthuer, Dominik (Author)
- Panneels, Valerie (Author)
- Cherezov, Vadim (Author)
- Chapman, Henry (Author)
- Schertler, Gebhard (Author)
- Neutze, Richard (Author)
- Spence, John (Author)
- Moraes, Isabel (Author)
- Burghammer, Manfred (Author)
- Standfuss, Joerg (Author)
- Weierstall, Uwe (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-01-27
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Identifier
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Digital object identifier: 10.1107/S2052252514026487
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Identifier TypeInternational standard serial numberIdentifier Value2052-2525
Note
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View the article as published at http://journals.iucr.org/m/issues/2015/02/00/jt5008/index.html
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Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Nogly, P., James, D., Wang, D., White, T. A., Zatsepin, N., Shilova, A., . . . Weierstall, U. (2015). Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. IUCrJ, 2(2), 168-176. doi:10.1107/s2052252514026487