Description
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
Details
Title
- Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
Contributors
- Fromme, Raimund (Author)
- Ishchenko, Andrii (Author)
- Metz, Markus (Author)
- Roy Chowdhury, Shatabdi (Author)
- Basu, Shibom (Author)
- Boutet, Sebastien (Author)
- Fromme, Petra (Author)
- White, Thomas A. (Author)
- Barty, Anton (Author)
- Spence, John (Author)
- Weierstall, Uwe (Author)
- Liu, Wei (Author)
- Cherezov, Vadim (Author)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-08-04
Resource Type
Collections this item is in
Identifier
- Digital object identifier: 10.1107/S2052252515013160
- Identifier TypeInternational standard serial numberIdentifier Value2052-2525
Note
- View the article as published at http://journals.iucr.org/m/issues/2015/05/00/lz5008/index.html
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Fromme, R., Ishchenko, A., Metz, M., Chowdhury, S. R., Basu, S., Boutet, S., . . . Cherezov, V. (2015). Serial femtosecond crystallography of soluble proteins in lipidic cubic phase. IUCrJ, 2(5), 545-551. doi:10.1107/s2052252515013160