Description
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
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Title
- Serial femtosecond X-ray diffraction of enveloped virus microcrystals
Contributors
- Lawrence, Robert (Author)
- Conrad, Chelsie (Author)
- Zatsepin, Nadia (Author)
- Grant, Thomas D. (Author)
- Liu, Haiguang (Author)
- James, Daniel (Author)
- Nelson, Garrett (Author)
- Subramanian, Ganesh (Author)
- Aquila, Andrew (Author)
- Hunter, Mark S. (Author)
- Liang, Mengning (Author)
- Boutet, Sebastien (Author)
- Coe, Jesse (Author)
- Spence, John (Author)
- Weierstall, Uwe (Author)
- Liu, Wei (Author)
- Fromme, Petra (Author)
- Cherezov, Vadim (Author)
- Hogue, Brenda (Author)
- Biodesign Institute (Contributor)
- Infectious Diseases and Vaccinology (Contributor)
- Applied Structural Discovery (Contributor)
- Department of Chemistry and Biochemistry (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
- School of Life Sciences (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-08-20
Resource Type
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Identifier
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Digital object identifier: 10.1063/1.4929410
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Identifier TypeInternational standard serial numberIdentifier Value2329-7778
Citation and reuse
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Lawrence, R. M., Conrad, C. E., Zatsepin, N. A., Grant, T. D., Liu, H., James, D., . . . Hogue, B. G. (2015). Serial femtosecond X-ray diffraction of enveloped virus microcrystals. Structural Dynamics, 2(4), 041720. doi:10.1063/1.4929410