Description
Serial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of “diffraction-before-destruction.” However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A[subscript 2A] adenosine receptor at 1.9 Å resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes.
Details
Title
- Native phasing of x-ray free-electron laser data for a G protein–coupled receptor
Contributors
- Batyuk, Alexander (Author)
- Galli, Lorenzo (Author)
- Ishchenko, Andrii (Author)
- Han, Gye Won (Author)
- Gati, Cornelius (Author)
- Popov, Petr A. (Author)
- Lee, Ming-Yue (Author)
- Stauch, Benjamin (Author)
- White, Thomas A. (Author)
- Barty, Anton (Author)
- Aquila, Andrew (Author)
- Hunter, Mark S. (Author)
- Liang, Mengning (Author)
- Boutet, Sebastien (Author)
- Pu, Mengchen (Author)
- Liu, Zhi-jie (Author)
- Nelson, Garrett (Author)
- James, Daniel (Author)
- Li, Chufeng (Author)
- Zhao, Yun (Author)
- Spence, John (Author)
- Liu, Wei (Author)
- Fromme, Petra (Author)
- Katritch, Vsevolod (Author)
- Weierstall, Uwe (Author)
- Stevens, Raymond C. (Author)
- Cherezov, Vadim (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- School of Molecular Sciences (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2016-09-23
Resource Type
Collections this item is in
Identifier
- Digital object identifier: 10.1126/sciadv.1600292
- Identifier TypeInternational standard serial numberIdentifier Value2375-2548
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Batyuk, A., Galli, L., Ishchenko, A., Han, G. W., Gati, C., Popov, P. A., . . . Cherezov, V. (2016). Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Science Advances, 2(9). doi:10.1126/sciadv.1600292