Full metadata
Title
Electronic single-molecule identification of carbohydrate isomers by recognition tunnelling
Description
Carbohydrates are one of the four main building blocks of life, and are categorized as monosaccharides (sugars), oligosaccharides and polysaccharides. Each sugar can exist in two alternative anomers (in which a hydroxy group at C-1 takes different orientations) and each pair of sugars can form different epimers (isomers around the stereocentres connecting the sugars). This leads to a vast combinatorial complexity, intractable to mass spectrometry and requiring large amounts of sample for NMR characterization. Combining measurements of collision cross section with mass spectrometry (IM–MS) helps, but many isomers are still difficult to separate. Here, we show that recognition tunnelling (RT) can classify many anomers and epimers via the current fluctuations they produce when captured in a tunnel junction functionalized with recognition molecules. Most importantly, RT is a nanoscale technique utilizing sub-picomole quantities of analyte. If integrated into a nanopore, RT would provide a unique approach to sequencing linear polysaccharides.
Date Created
2016-12-21
Contributors
- Im, Jong One (Author)
- Biswas, Sovan (Author)
- Liu, Hao (Author)
- Zhao, Yanan (Author)
- Sen, Suman (Author)
- Biswas, Sudipta (Author)
- Ashcroft, Brian (Author)
- Borges, Chad (Author)
- Wang, Xu (Author)
- Lindsay, Stuart (Author)
- Zhang, Peiming (Author)
- Biodesign Institute (Contributor)
- Single Molecule Biophysics (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
- School of Molecular Sciences (Contributor)
Resource Type
Extent
7 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.1038/ncomms13868
Identifier Type
International standard serial number
Identifier Value
2041-1723
Series
NATURE COMMUNICATIONS
Handle
https://hdl.handle.net/2286/R.I.44786
Preferred Citation
Im, J., Biswas, S., Liu, H., Zhao, Y., Sen, S., Biswas, S., . . . Zhang, P. (2016). Electronic single-molecule identification of carbohydrate isomers by recognition tunnelling. Nature Communications, 7, 13868. doi:10.1038/ncomms13868
Level of coding
minimal
Cataloging Standards
Note
The final version of this article, as published in Nature Communications, can be viewed online at: http://aca.scitation.org/doi/10.1063/1.4919398
System Created
- 2017-07-12 04:46:47
System Modified
- 2021-08-16 02:23:30
- 3 years 1 month ago
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