Full metadata
Title
XFELs for structure and dynamics in biology
Description
The development and application of the free-electron X-ray laser (XFEL) to structure and dynamics in biology since its inception in 2009 are reviewed. The research opportunities which result from the ability to outrun most radiation-damage effects are outlined, and some grand challenges are suggested. By avoiding the need to cool samples to minimize damage, the XFEL has permitted atomic resolution imaging of molecular processes on the 100 fs timescale under near-physiological conditions and in the correct thermal bath in which molecular machines operate. Radiation damage, comparisons of XFEL and synchrotron work, single-particle diffraction, fast solution scattering, pump–probe studies on photosensitive proteins, mix-and-inject experiments, caged molecules, pH jump and other reaction-initiation methods, and the study of molecular machines are all discussed. Sample-delivery methods and data-analysis algorithms for the various modes, from serial femtosecond crystallography to fast solution scattering, fluctuation X-ray scattering, mixing jet experiments and single-particle diffraction, are also reviewed.
Date Created
2017-05-10
Contributors
- Spence, John (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
Resource Type
Extent
18 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.1107/S2052252517005760
Identifier Type
International standard serial number
Identifier Value
2052-2525
Series
IUCrJ
Handle
https://hdl.handle.net/2286/R.I.45071
Preferred Citation
Spence, J. C. (2017). XFELs for structure and dynamics in biology. IUCrJ, 4(4), 322-339. doi:10.1107/s2052252517005760
Level of coding
minimal
Cataloging Standards
Note
View the article as published at http://journals.iucr.org/m/issues/2017/04/00/hi5644/index.html
System Created
- 2017-08-09 12:04:37
System Modified
- 2021-08-16 02:23:30
- 3 years 2 months ago
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