Description
X-ray diffraction patterns from two-dimensional (2-D) protein crystals obtained using femtosecond X-ray pulses from an X-ray free-electron laser (XFEL) are presented. To date, it has not been possible to acquire transmission X-ray diffraction patterns from individual 2-D protein crystals due to radiation damage. However, the intense and ultrafast pulses generated by an XFEL permit a new method of collecting diffraction data before the sample is destroyed. Utilizing a diffract-before-destroy approach at the Linac Coherent Light Source, Bragg diffraction was acquired to better than 8.5 Å resolution for two different 2-D protein crystal samples each less than 10 nm thick and maintained at room temperature. These proof-of-principle results show promise for structural analysis of both soluble and membrane proteins arranged as 2-D crystals without requiring cryogenic conditions or the formation of three-dimensional crystals.
Details
Title
- Femtosecond X-ray diffraction from two-dimensional protein crystals
Contributors
- Frank, Matthias (Author)
- Carlson, David B. (Author)
- Hunter, Mark S. (Author)
- Williams, Garth J. (Author)
- Messerschmidt, Marc (Author)
- Zatsepin, Nadia (Author)
- Barty, Anton (Author)
- Benner, W. Henry (Author)
- Chu, Kaiqin (Author)
- Graf, Alexander T. (Author)
- Hau-Riege, Stefan P. (Author)
- Kirian, Richard A. (Author)
- Padeste, Celestino (Author)
- Pardini, Tommaso (Author)
- Pedrini, Bill (Author)
- Segelke, Brent (Author)
- Seibert, M. Marvin (Author)
- Spence, John (Author)
- Tsai, Ching-Ju (Author)
- Lane, Stephen M. (Author)
- Li, Xiao-Dan (Author)
- Schertler, Gebhard (Author)
- Boutet, Sebastien (Author)
- Coleman, Matthew (Author)
- Evans, James E. (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2014-02-28
Resource Type
Collections this item is in
Identifier
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Digital object identifier: 10.1107/S2052252514001444
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Identifier TypeInternational standard serial numberIdentifier Value2052-2525
Note
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View the article as published at http://journals.iucr.org/m/issues/2014/02/00/cw5002/index.html
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Frank, M., Carlson, D. B., Hunter, M. S., Williams, G. J., Messerschmidt, M., Zatsepin, N. A., . . . Evans, J. E. (2014). Femtosecond X-ray diffraction from two-dimensional protein crystals. IUCrJ, 1(2), 95-100. doi:10.1107/s2052252514001444