Description
X-ray free-electron lasers (XFELs) provide new opportunities for structure determination of biomolecules, viruses and nanomaterials. With unprecedented peak brilliance and ultra-short pulse duration, XFELs can tolerate higher X-ray doses by exploiting the femtosecond-scale exposure time, and can thus go beyond the resolution limits achieved with conventional X-ray diffraction imaging techniques. Using XFELs, it is possible to collect scattering information from single particles at high resolution, however particle heterogeneity and unknown orientations complicate data merging in three-dimensional space. Using the Linac Coherent Light Source (LCLS), synthetic inorganic nanocrystals with a core–shell architecture were used as a model system for proof-of-principle coherent diffractive single-particle imaging experiments. To deal with the heterogeneity of the core–shell particles, new computational methods have been developed to extract the particle size and orientation from the scattering data to assist data merging. The size distribution agrees with that obtained by electron microscopy and the merged data support a model with a core–shell architecture.
Details
Title
- Merging single-shot XFEL diffraction data from inorganic nanoparticles: a new approach to size and orientation determination
Contributors
- Li, Xuanxuan (Author)
- Spence, John (Author)
- Hogue, Brenda (Author)
- Liu, Haiguang (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2017-08-27
Resource Type
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Identifier
- Digital object identifier: 10.1107/S2052252517012398
- Identifier TypeInternational standard serial numberIdentifier Value2052-2525
Note
- View the article as published at http://journals.iucr.org/m/issues/2017/06/00/it5013/index.html
Citation and reuse
Cite this item
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Li, X., Spence, J. C., Hogue, B. G., & Liu, H. (2017). Merging single-shot XFEL diffraction data from inorganic nanoparticles: a new approach to size and orientation determination. IUCrJ, 4(6), 741-750. doi:10.1107/s2052252517012398