Quantifying the interactions of bacteria with external ligands is fundamental to the understanding of pathogenesis, antibiotic resistance, immune evasion, and mechanism of antimicrobial action. Due to inherent cell-to-cell heterogeneity in a microbial population, each bacterium interacts differently with its environment. This large variability is washed out in bulk assays, and there is a need of techniques that can quantify interactions of bacteria with ligands at the single bacterium level. In this work, we present a label-free and real-time plasmonic imaging technique to measure the binding kinetics of ligand interactions with single bacteria, and perform statistical analysis of the heterogeneity. Using the technique, we have studied interactions of antibodies with single Escherichia coli O157:H7 cells and demonstrated a capability of determining the binding kinetic constants of single live bacteria with ligands, and quantify heterogeneity in a microbial population.
Details
- Plasmonic Imaging of Protein Interactions With Single Bacterial Cells
- Syal, Karan (Author)
- Wang, Wei (Author)
- Shan, Xiaonan (Author)
- Wang, Shaopeng (Author)
- Chen, Hong-Yuan (Author)
- Tao, Nongjian (Author)
- Biodesign Institute (Contributor)
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Digital object identifier: 10.1016/j.bios.2014.06.069
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Identifier TypeInternational standard serial numberIdentifier Value0093-934X
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Identifier TypeInternational standard serial numberIdentifier Value1090-2155
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NOTICE: this is the author's version of a work that was accepted for publication in BIOSENSORS & BIOELECTRONICS. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in BIOSENSORS & BIOELECTRONICS, 63, 131-137. DOI: 10.1016/j.bios.2014.06.069
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Syal, Karan, Wang, Wei, Shan, Xiaonan, Wang, Shaopeng, Chen, Hong-Yuan, & Tao, Nongjian (2015). Plasmonic imaging of protein interactions with single bacterial cells. BIOSENSORS & BIOELECTRONICS, 63, 131-137. http://dx.doi.org/10.1016/j.bios.2014.06.069