Effective DNA translocation into nanochannels is critical for advancing genome mapping and future single-molecule DNA sequencing technologies. We present the design and hydrodynamic study of a diamond-shaped gradient pillar array connected to nanochannels for enhancing the success of DNA translocation events. Single-molecule fluorescence imaging is utilized to interrogate the hydrodynamic interactions of the DNA with this unique structure, evaluate key DNA translocation parameters, including speed, extension, and translocation time, and provide a detailed mapping of the translocation events in nanopillar arrays coupled with 10 and 50 μm long channels. Our analysis reveals the important roles of diamond-shaped nanopillars in guiding DNA into as small as 30 nm channels with minimized clogging, stretching DNA to nearly 100% of their dyed contour length, inducing location-specific straddling of DNA at nanopillar interfaces, and modulating DNA speeds by pillar geometries. Importantly, all critical features down to 30 nm wide nanochannels are defined using standard photolithography and fabrication processes, a feat aligned with the requirement of high-volume, low-cost production.
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- Hydrodynamics of Diamond-Shaped Gradient Nanopillar Arrays for Effective DNA Trans location Into Nano Channels
- Wang, Chao (Author)
- Bruce, Robert L. (Author)
- Duch, Elizabeth A. (Author)
- Patel, Jyotica V. (Author)
- Smith, Joshua T. (Author)
- Astier, Yann (Author)
- Wunsch, Benjamin H. (Author)
- Meshram, Siddharth (Author)
- Galan, Armand (Author)
- Scerbo, Chris (Author)
- Pereira, Michael A. (Author)
- Wang, Deqiang (Author)
- Colgan, Evan G. (Author)
- Lin, Qinghuang (Author)
- Stolovitzky, Gustavo (Author)
- Ira A. Fulton Schools of Engineering (Contributor)
- Digital object identifier: 10.1021/nn507350e
- Identifier TypeInternational standard serial numberIdentifier Value1936-0851
- Identifier TypeInternational standard serial numberIdentifier Value1936-086X
- This document is the Accepted Manuscript version of a Published Work that appeared in final form in
ACS NANO, copyright American Chemical Society, after peer review and technical editing by the publisher.
To access the final published work, see http://dx.doi.org/10.1021/nn507350e
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Wang, Chao, Bruce, Robert L., Duch, Elizabeth A., Patel, Jyotica V., Smith, Joshua T., Astier, Yann, Wunsch, Benjamin H., Meshram, Siddharth, Galan, Armand, Scerbo, Chris, Pereira, Michael A., Wang, Deqiang, Colgan, Evan G., Lin, Qinghuang, & Stolovitzky, Gustavo (2015). Hydrodynamics of Diamond-Shaped Gradient Nanopillar Arrays for Effective DNA Trans location into Nano channels. ACS NANO, 9(2), 1206-1218. http://dx.doi.org/10.1021/nn507350e