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Functional and molecular cell-to-cell variability is pivotal at the cellular, tissue and whole-organism levels. Yet, the ultimate goal of directly correlating the function of the individual cell with its biomolecular profile remains elusive. We present a platform for integrated analysis

Functional and molecular cell-to-cell variability is pivotal at the cellular, tissue and whole-organism levels. Yet, the ultimate goal of directly correlating the function of the individual cell with its biomolecular profile remains elusive. We present a platform for integrated analysis of functional and transcriptional phenotypes in the same single cells. We investigated changes in the cellular respiration and gene expression diversity resulting from adaptation to repeated episodes of acute hypoxia in a premalignant progression model. We find differential, progression stage-specific alterations in phenotypic heterogeneity and identify cells with aberrant phenotypes. To our knowledge, this study is the first demonstration of an integrated approach to elucidate how heterogeneity at the transcriptional level manifests in the physiologic profile of individual cells in the context of disease progression.

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    Title
    • Platform for Combined Analysis of Functional and Biomolecular Phenotypes of the Same Cell
    Contributors
    Date Created
    2017-03-16
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.1038/srep44636
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
    Note
    • The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep44636

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    Kelbauskas, L., Ashili, S., Zeng, J., Rezaie, A., Lee, K., Derkach, D., . . . Meldrum, D. R. (2017). Platform for combined analysis of functional and biomolecular phenotypes of the same cell. Scientific Reports, 7, 44636. doi:10.1038/srep44636

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