Description

We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors

We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2, the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.

Reuse Permissions
  • Downloads
    PDF (1.1 MB)

    Details

    Title
    • Serial Femtosecond Crystallography Datasets From G Protein-Coupled Receptors
    Contributors
    Date Created
    2016-08-01
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1038/sdata.2016.57
    • Identifier Type
      International standard serial number
      Identifier Value
      2052-4463
    Note
    • The final version of this article, as published in Scientific Data, can be viewed online at: https://www.nature.com/articles/sdata201657

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    White, T. A., Barty, A., Liu, W., Ishchenko, A., Zhang, H., Gati, C., . . . Cherezov, V. (2016). Serial femtosecond crystallography datasets from G protein-coupled receptors. Scientific Data, 3, 160057. doi:10.1038/sdata.2016.57

    Machine-readable links