Description

In carcinogenesis, intercellular interactions within and between cell types are critical but remain poorly understood. We present a study on intercellular interactions between normal and premalignant epithelial cells and their functional relevance in the context of premalignant to malignant progression

In carcinogenesis, intercellular interactions within and between cell types are critical but remain poorly understood. We present a study on intercellular interactions between normal and premalignant epithelial cells and their functional relevance in the context of premalignant to malignant progression in Barrett’s esophagus. Using whole transcriptome profiling we found that in the presence of normal epithelial cells, dysplastic cells but not normal cells, exhibit marked down-regulation of a number of key signaling pathways, including the transforming growth factor beta (TGFβ) and epithelial growth factor (EGF). Functional assays revealed both cell types showed repressed proliferation and significant changes in motility (speed, displacement and directionality) as a result of interactions between the two cell types. Cellular interactions appear to be mediated through both direct cell-cell contact and secreted ligands. The findings of this study are important in that they reveal, for the first time, the effects of cellular communication on gene expression and cellular function between premalignant (dysplastic) epithelial cells and their normal counterparts.

Reuse Permissions
  • Downloads
    PDF (2 MB)

    Details

    Title
    • Transcriptional Regulation by Normal Epithelium of Premalignant to Malignant Progression in Barrett’s Esophagus
    Contributors
    Date Created
    2016-10-12
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1038/srep35227
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
    Note
    • The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/srep35227

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Zeng, J., Kelbauskas, L., Rezaie, A., Lee, K., Ueberroth, B., Gao, W., . . . Meldrum, D. R. (2016). Transcriptional regulation by normal epithelium of premalignant to malignant progression in Barrett’s esophagus. Scientific Reports, 6(1). doi:10.1038/srep35227

    Machine-readable links