Description

The oxidative modification of apolipoprotein A-I’s methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to quantitate post-translational modifications. In this study, we developed an MRM

The oxidative modification of apolipoprotein A-I’s methionine148 (M148) is associated with defective HDL function in vitro. Multiple reaction monitoring (MRM) is a mass spectrometric technique that can be used to quantitate post-translational modifications. In this study, we developed an MRM assay to monitor the abundance ratio of the peptide containing oxidized M148 to the native peptide in ApoA-I. Measurement of the oxidized-to-unoxidized-M148 ratio was reproducible (CV < 5%). The extent of methionine M148 oxidation in the HDL of healthy controls, and type 2 diabetic participants with and without prior cardiovascular events (CVD) were then examined. The results suggest a significant increase in the relative ratio of the peptide containing oxidized M148 to the unmodified peptide in the HDL of participants with diabetes and CVD (p < 0.001), compared to participants without CVD. Monitoring the abundance ratio of the peptides containing oxidized and unoxidized M148 by MRM provides a means of examining the relationship between M148 oxidation and vascular complications in CVD.

Reuse Permissions
  • Downloads
    PDF (772.9 KB)

    Details

    Title
    • The Application of Multiple Reaction Monitoring to Assess ApoA-I Methionine Oxidations in Diabetes and Cardiovascular Disease
    Contributors
    Date Created
    2014-10-11
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1016/j.trprot.2014.10.001
    • Identifier Type
      International standard serial number
      Identifier Value
      2212-9626
    • Identifier Type
      International standard serial number
      Identifier Value
      2212-9634
    Note
    • The final version of this article, as published in Translational Proteomics, can be viewed online at: http://www.sciencedirect.com/science/article/pii/S2212963414000102?via%3Dihub

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Yassine, H. N., Jackson, A. M., Reaven, P. D., Nedelkov, D., Nelson, R. W., Lau, S. S., & Borchers, C. H. (2014). The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease. Translational Proteomics, 4-5, 18-24. doi:10.1016/j.trprot.2014.10.001

    Machine-readable links